Strand exchange amplification
Web14 Jun 2024 · Herein, a simple, rapid, and effective method, accelerated strand exchange amplification (ASEA), was developed for EBV detection, which could offer a strategy for non-invasive testing of EBV in saliva samples instead of blood samples as in traditional serology based methods and avoid bleeding during diagnosis. Web22 Mar 2024 · Here, we established a two-stage isothermal amplification method comprising strand exchange amplification and hyperbranched rolling circle amplification, which only employed one DNA polymerase and was performed in a single closed tube at a constant temperature, providing a promising signal amplification strategy for accurate …
Strand exchange amplification
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Web11 Feb 2024 · Salmonella is one of the most common pathogens associated with food-borne illness resulting from seafood consumption. Herein, an accelerated strand exchange amplification (ASEA) requiring only a pair of primers and one polymerase was first reported for ultra-fast, one-step RNA amplification detection of Salmonella in seafood. The ASEA …
WebIn this study, we developed a visual strand exchange amplification detection assay for ASFV. In the presence of ASFV, DNA amplification products containing multimeric G … Web23 Feb 2015 · Loop-mediated isothermal amplification (LAMP) of DNA is a powerful isothermal nucleic acid amplification method that can generate upward of 109 copies from less than 100 copies of template DNA within an hour. Unfortunately, although the amplification reactions are extremely powerful, real-time and specific detection of LAMP …
Web3 Feb 2024 · H. pylori can be isolated from human saliva, and the sampling of saliva is easy and convenient. Therefore, we developed a visual denaturation bubble-mediated strand … Web9 May 2024 · In this work, we established and evaluated a graphene oxide-based accelerated strand exchange amplification (GO-ASEA) method for rapid, highly sensitive, and …
Web10 Apr 2024 · ATRX promotes DNA repair synthesis and sister chromatid exchange HR . DNA double-strand breaks (DSBs) occur when cells are exposed to endogenous or exogenous stress. DSBs can be repaired by two ... Amplification of the MYCN oncogene and inactivation of the ATRX tumor-suppressor gene are associated with high-risk disease and …
WebWe used a denaturation bubble-mediated strand exchange amplification (SEA) method to successfully detect CPV-2 and FPLV in fecal samples. SEA could detect as little as 3.6 … culver academies history vaultWeb28 Mar 2024 · In this study, we developed a staggered strand exchange amplification (SSEA) method by refining denaturation bubble-mediated strand exchange amplification (SEA) to … culver academy basketball twitterWeb15 Aug 2024 · Strand exchange amplification (SEA) is a simple isothermal amplification method [ 32 ], it takes advantage of the DNA duplex's dynamically dissociate at appropriate reaction temperature, leading the local opening of base pairs to create a single-stranded denaturation bubble [ [33], [34], [35]]. east moline mayorWeb21 Feb 2024 · INTRODUCTION. Homologous recombination (HR) is a DNA strands exchange process essential for multiple pathways of genome maintenance and plasticity in all kingdoms of life (1, 2).Defects in any of these pathways lead to deleterious consequences, such as cell death or various types of cancer ().HR relies on the pairing of a single … culver academy athletic directorWeb15 Mar 2024 · Abstract Denaturation bubble-mediated strand exchange amplification (SEA) is a novel, rapid isothermal nucleic acid amplification has been applied for point-of-care … culver academy cross country invitationalWeb17 Mar 2015 · In order to both improve the specificity of LAMP detection and to make readout simpler and more reliable, we have replaced the intercalating dye typically used for monitoring in real-time fluorescence with a toehold-mediated strand exchange reaction termed one-step strand displacement (OSD). culver academy battery boards quizletWeb8 Nov 2024 · Denaturation Bubble-mediated Strand Exchange Amplification (SEA) was chosen to perform isothermal amplification because it requires only a pair of primers and one Bst DNA polymerase. The established SEA can detect as low as 1.0 × 10-13 M genomic DNA of B. cereus, which was comparable with the previously reported method for B. … culver academy application