Pcpgfree-mcs
Splet18. okt. 2016 · CpG carrying versions of the promoter was cloned from the pCpGrich-mcs vector (InvivoGen, San Diego, CA) and the CpG free version was cloned from pCpGfree … SpletpCpGfree-promoter is a reporter plasmid completely devoid of CpG dinucleotides. This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase …
Pcpgfree-mcs
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SpletInvivoGen cpg free plasmid Cpg Free Plasmid, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more SpletpCpGfree-vitro is a new family of expression vectors completely devoid of CpG dinucleotides that are selectable in mammalian cells. Similarly to the other pCpGfree …
SpletTDS pCpGfree-mcs - InvivoGen. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi … Splet28. jun. 2012 · The open-read frame of RGD4C-hTNF-α was integrated at the multi-cloning sites in the plasmid DNA of pVIVO1-mcs with hamster 78-kDa glucose-regulated protein (GRP78) promoter which yields persistent high expression within the tumor micro-environment or in the plasmid of pCpGfree-mcs to delete immunogenic CpG motifs with …
Splet3’) was cloned into a CpG-free vector (pCpGfree-mcs Invivogen, San Diego, CA, USA) using BglII and NcoI restriction endonuclease sites (New England Biolabs Ltd., Pickering, ON, Canada). The gene was designed such that it had minimal CpG oligodeoxynucleotides and was optimized for high expression in Splet01. nov. 2013 · To prepare pDNA encoding luciferase that is completely free from unmethylated CpG dinucleotide motifs (pCpGfree-Luc (0)), the multiple cloning site of pCpGfree-mcs (Invivogen, San Diego, CA, USA) was preliminarily replaced with a new one that can be digested by 5′-Bgl II-Pvu II-Nco I-Sca I-Xba I-Nhe 1-3′ by ligating the hybridized …
SpletpCpGfree-vitro is a family of expression vectors devoid of CpG dinucleotides that are selectable in mammalian cells. All the elements required for replication and selection of …
Splet28. feb. 2015 · To prepare pDNA encoding luciferase that is completely free from unmethylated CpG dinucleotide motifs (pCpGfree-Luc(0)), the multiple cloning site of pCpGfree-mcs (Invivogen, San Diego, CA, USA) was preliminarily replaced with a new one that can be digested by 5′-Bgl II-Pvu II-Nco I-Sca I-Xba I-Nhe 1-3′ by ligating the hybridized ... port of orleans french quarterSpletpCpGfree-mcs contains an MCS featuring several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT … iron hive makers cultSpletpCpGfree-mcs. Similar products. Human MCS CRISPR Knock Out 293 Cell Line. Catalog number: K1282152. Size: 1x10^6 cells/1.0ml. Price: Please ask. Human MCS CRISPR … iron hingesSpletpCpGrich-mcs is a CpG-containing control plasmid. The CpG-free version of the murine CMV enhancer, human EF-1α promoter, ori R6K gamma, bacterial EM2K promoter and … port of oswego grocery storesSpletCpGs) and a pCpGfree-mcs vec- tor encoding the CpG-positive luciferase gene (pCpGfree- Luc; 93 CpGs). The removal of the CpG sequences from the backbone and/or the ORF … iron hno3Splet22. jul. 2015 · The Luc-GFP cassette was cloned by PCR cloning into pCpG F using NheI and BglII ends in order to produce the same expression cassette (CMV enhancer and EF1 promoter) in pCpG F. Vectors were prepared for all experiments using Endofree kits (Qiagen, Valencia, CA, USA). iron hindiSpletExpression vector CpG-free with MCS, LacZ or mSEAP pCpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication … port of oshawa ontario