2024 Hepg2 dual

Hepg2 dual

Author: ytpp

August undefined, 2024

WebHB-8065 ™. Hep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver …

A dual near-infrared pH fluorescent probe and its application in ...

WebHepG2 cells were treated by 200 μM H2O2 with or without a series of concentrations of NAS ... which is confirmed by the appearance of apoptotic bodies with PI and AO/EB dual staining, ... Web1 giu 2024 · These dual-functional immune-reagents have great potential in biosensors developing for diagnosis application. Abstract. ... Liver cancer cell lines, such as HepG2, Huh-7, Hep3B, SMMC-7721, SK-Hep-1cells were incubated with nanobody individually in PBS (2% BSA) buffer for 30 min with gentle shaking. After washing with PBS, ... civil division us attorney office

Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 ...

WebHep G2 cells are passaged 24 hr prior to transfection to give a cell density of 50-60% confluence at the time of transfection. The adherent cells are washed in 1 x HBS, and … Web1 lug 2024 · In view of properties of the two antibodies (anti-CD63 and anti-EpCAM), we demonstrate a dual antibody-modified nanochannel biosensor for label-free, rapid, highly sensitive detection of exosomes. Web19 set 2024 · Briefly, HepG2 cells or DGKθ-T2A-Luciferase knock-in HepG2 cells were plated on a 24-well plate at a density of 2 × 10 5 cells per well for 24 h followed by the treatment with Bt 2 cAMP ... the cells were harvested and the transcriptional activity of the hDGKθ reporter gene was measured using a dual luciferase assay kit (Promega). douglas shorenstein

Enhancing cisplatin delivery to hepatocellular carcinoma HepG2 …

WebHepG2-Dual™ cells were stimulated with numerous ligands. None of the TLR ligands tested induced an NF-κB or IRF response, with the exception of the TLR2 ligand Pam3CSK4, … Web7 ago 2014 · Thus, we created transgenic HepaRG and HepG2 cells by replacing the protein-coding regions of human CYP3A4 and CYP3A7 with enhanced green fluorescent protein (EGFP) and DsRed reporters, respectively, ... Dual-Color Fluorescence Imaging to Monitor CYP3A4 and CYP3A7 Expression in Human Hepatic Carcinoma HepG2 and … civil docket search in delaware countyWebInvivoGen provides HepG2 cell lines engineered to express the secreted embryonic alkaline phosphatase (SEAP) and Lucia luciferase reporter proteins for assessing the induction of … civil docket search

"Web1 giu 2015 · Overall, we have demonstrated that HepG2 cells acutely deprived of glucose are a suitable model for the identification of mitochondrial toxicity when used alongside … " - Hepg2 dual

Hepg2 dual

WebHepG2-Dual: Accession: CVCL_A8BS: Resource Identification Initiative: To cite this cell line use: HepG2-Dual (RRID:CVCL_A8BS) Comments: Population: Caucasian. … Web15 feb 2024 · A novel dual-locked NIR fluorescent probe ( DLSP) was designed and successfully synthesized. • DLSP could selectively visualize the biosynthesis of endogenous H 2 S production in insulin-resistant HepG2 cells. • DLSP could be used to monitor the fluctuation of H 2 S in diabetic mice. Keywords 1. Introduction

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Web1 nov 2024 · Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H 2 S/viscosity in the brain of drosophila Parkinson’s disease model. ... WebDDIT3 Directs a Dual Mechanism to Balance Glycolysis and Oxidative Phosphorylation during Glutamine Deprivation. Mingyue Li, ... HepG2 cells transfected with either Flag control or Flag-DDIT3 were added protease inhibitor cocktail 1:25 before cell lysis and immunoprecipitations performed using control IgG (IgG-R) or anti-COQ9.

Web14 apr 2024 · Dual PTEN/p53 deficiency can also stabilize HK2 mRNA through post-transcriptional and translational regulation via inhibition of miR143 biogenesis ... SESN2, and SESN3 in the HepG2 HCC cell line. Web11 apr 2024 · Dual luciferase reporter gene assay showed that miR-146a-5p can directly target FLAP mRNA. Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion.

Web5 apr 2024 · In this study, the novel multifunctional liposomes (CAPS-CUR/GA&Gal-Lip) were prepared for co-delivery of curcumin (CUR) and capsaicin (CAPS), in which glycyrrhetinic acid (GA) and galactose (Gal) were chosen as targeting ligands to modify the liposomes (Lip) for dual-targeting liver cancer. Web4 dic 2024 · HepG2 is a popular hepatic cell line. It is used in a wide range of studies, from the oncogenesis to the cytotoxicity of substances on the liver. In this regard, we set out …

WebOverall, these studies demonstrate the utility of HepG2 cells for the identification of mitochondrial toxins which act directly on the electron transport chain and that the dual …

Web25 set 2024 · Conclusion: Fluorescence-quenched nanoparticle NIR-CBT-NP was facilely prepared to actively target biotin receptor-overexpressing HepG2 cancer cells and turn … civil - domestic case information reportWeb12 gen 2024 · Dual Agonist of Farnesoid X Receptor and Takeda G Protein-Coupled Receptor 5 Inhibits Hepatitis B ... -isopropylisoxazole (GW4064; a FXR agonist), cyclosporin A, and irbesartan. OCA and INT-777 … civil domestic information reportWeb15 feb 2024 · Monitoring the fluctuation of H 2 S in insulin-resistant HepG2 cells and diabetic mice with a dual-locked NIR fluorescent probe. Author links open overlay panel Zimeng … civil docket search pike county paWebIn the present study, it was shown that IL-18 and its receptors (IL-18R) were constitutively expressed in hepatoma cell lines HepG2 and HepG2.2.15 as well as normal liver cell line HL-7702. We demonstrated that IL-18 directly inhibited HBV replication in HepG2.2.15 cells via downregulating the activities of HBV core and X gene promoters. civil docket search travis county texasWeb1 set 2024 · After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. ... which led to the upregulation of dual-specificity phosphatase-9 (DUSP9), a direct target of miR-212, ... civil docket search in lehigh county paWeb11 mar 2015 · In HepG2 model, HN3-PE38 alone was less effective. As a single agent, 0.6 mg kg −1 HN3-PE38 only delayed HepG2 tumour growth. This was probably due to the different cell context of HepG2 in ... civil docket travis countyWebThis dual-mode imaging strategy facilitates visualization of CTR1 migration and meanwhile provides information of CTR1 redistribution in HepG2 cells by uptake of divalent copper … douglas shopping night