Gatewaytm lr clonasetm ii enzyme mix 说明书
WebGateway® LR Clonase™ II Enzyme Mix 20 reactions 11791-020 100 reactions 11791-100 Gateway® LR Clonase™ Plus Enzyme Mix 20 reactions 12538-013 MultiSite Gateway® Three-Fragment Vector Construction Kit 1 kit 12537-023 AcTEV™ Protease 1,000 units 12575-015 10,000 units 12575-023 WebLR Clonase II Plus 酶混合物是最新的产品型号，可提供最高的重组效率（表 1），并专门针对单片段和多片段克隆进行了优化（表 1）。 LR Clonase II Plus 为包含酶和反应缓冲液的优化型单一酶混合物，进行单片段和多片段克隆时所需的步骤更少，因此可以确保酶的稳定 ...
Gatewaytm lr clonasetm ii enzyme mix 说明书
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WebGateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone. … WebGateway Lr Clonase Ii Plus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... lr clonase ii enzyme mix kit (Thermo Fisher) Thermo Fisher is a verified supplier Thermo Fisher manufactures this product . About; News ...
Web• LR Clonase® II Plus enzyme mix (keep at –20°C until immediately before use) • 1X TE Buffer, pH 8.0 (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) • 2 μg/μL Proteinase K solution (supplied with the enzyme mix; thaw and keep on ice until use) • Appropriate competent E. coli host and growth media for expression • S.O.C. Medium
WebNov 9, 2024 · Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a … WebSome drug abuse treatments are a month long, but many can last weeks longer. Some drug abuse rehabs can last six months or longer. At Your First Step, we can help you to …
WebLR Clonase™ II Enzyme Mix The following reagents are supplied with the Gateway® LR Clonase™ II Enzyme Mix (Box 2). Store Box 2 at –20°C for up to 6 months. For long-term storage, keep at –80°C. Reagent Composition Amount LR Clonase™ II enzyme mix Proprietary 40 μl Proteinase K 2 mg/ml in: 10 mM Tris-HCl, pH 7.5 20 mM CaCl 2 50% ...
WebII enzyme mix (Catalog no. 11791-020) to ™ II enzyme mix combines the proprietary enzyme formulation and 5X LR Clonase™ Reaction Buffer previously supplied as separate components in LR Clonase ™ enzyme mix into an optimized single-tube format for easier set-up of the LR recombination reaction. Use the protocol below to perform the LR ... the lock shop fargo ndWebGateway® LR Clonase™ II Enzyme Mix The following reagents are included with the Gateway® LR Clonase™ II Enzyme Mix (Box 2). Store Box 2 at -20°C for up to 6 months. For long-term storage, store at -80°C. Reagent Composition Amount Gateway® LR Clonase™ II Enzyme Mix Proprietary 40 µl Proteinase K Solution 2 µg/ l in: 10 mM Tris … tickets qatar world cupWeb3. Add 2 μL of LR Clonase ™ II Plus Enzyme Mix to each MultiSite Gateway ™ Pro LR reaction, then briefly vortex twice to mix well. Briefly microcentrifuge. 4. Return the LR … the lock shop moscow idahoWeb3. Add 2 μL of LR Clonase ™ II Plus Enzyme Mix to each MultiSite Gateway ™ Pro LR reaction, then briefly vortex twice to mix well. Briefly microcentrifuge. 4. Return the LR Clonase ™ II Plus Enzyme Mix to −80°C storage immediately after use. 5. Incubate the reactions at 25°C for 16 hours. 6. Add 1 μL of the Proteinase K Solution to ... the lock shop moscowhttp://csrri.iit.edu/~howard/biol446/Lab4.lr_clonaseii_man.pdf the lock shop morton ilWebFawn Creek KS Community Forum. TOPIX, Facebook Group, Craigslist, City-Data Replacement (Alternative). Discussion Forum Board of Fawn Creek Montgomery … the lock pub chorleyWebThe attB × P reaction is mediated by Gateway ® BP Clonase ™ II enzyme mix; the attL × attR reaction is mediated by Gateway ® LR Clonase ™ II enzyme mix. ccdB is the F plasmid-encoded gene that inhibits growth of E. coli (2,3) and “gene” represents any DNA segment of interest (e.g. PCR product, cDNA, genomic DNA). Description tickets qualtrics